Inhibition of NF-KB activity in rabbit vascular smooth muscle cells by lovastatin

Nuclear factor NF-KB is believed to play an important role in regulating the production of matrix met-alloproteinases (MMPs), which induce atherosclerosis, restenosis and plaque rupture. We incubated rabbit vascular smooth muscle cells(RVSMCs)with 5 nmol/L lovastatin in the presence of IL-1-a and PDGFBB (20g/L, respectively) to study whether lovastatin inhibited NF-KB binding activity induced by IL-1 and PDGF. The NF-KB activity was detected by electrophoretic mobility shift assay (EMSA); MMP-1 and MMP-3 were measured by western blotting; and MMP-9 was detected by zymography. The result showed that lovastatin strongly reduced NF-KB activity upregulated by IL-1 combined with PDGF, and lovastatin also dose-dependently inhibited the expression of MMP-1, -3 and -9 induced by IL-1 and PDGF. It suggested that the beneficial effects of statins may extend to mechanisms beyond cholesterol reduction.     

Magnetoresistance and microstructure evolution upon rapid thermal annealing of giant magnetoresistive Co/Cu/Co/CoNbZr multilayers

The magnetoresistance (MR) variation of Co/Cu/Co/CoNbZr spin valves as a result of rapid thermal annealing has been investigated. MR ratio of 3.8% was obtained in the as-deposited sample and a considerable increase to 6.86% was observed in the 450°C×60 s treated sample. Microstructure studies show that the enhancement of MR ratio is a consequence of the nano-crystallization of amorphous CoNbZr soft layer. The nano-crystallized CoNbZr possess fine and dense microstructure and excellent electrical and soft magnetic properties which leads to the MR enhancement. With increasing annealing temperature or annealing time, interface roughness caused by rapid grain growth decrease the MR ratio rapidly. XRD studies imply that the interfusion of Cu atom into the Co layer is another possible degradation mechanism of Co/Cu/Co/CoNbZr spin valves at annealing temperature beyond 550°C.     

温差电致冷在光电倍增管上的应用

通过阐述光电倍增管探测性能与工作温度的关系,全面分析了引入致冷的必要性,此外详细叙述了水冷式光电倍增管致冷器的设计结构和工作原理,目的是通过使用温差电致冷器来降低光电倍增管阴极的温度,达到-30℃甚至更低。我们进行了多次单分子探测试验,结果表明,此法可以显著地降低阳极暗电流三个数量级以上,大幅度地提高光电倍增管的灵敏度。温差电致冷技术的引入,极大地改善了光电倍增管的探测性能,从而拓展了其应用领域。     

企业信息标准化工作的探讨

介绍了湘钢企业信息标准化工作情况 ,分析了企业实施信息标准化时存在的问题 ,需要采取的对策及其效果。     

PLC故障检测与日常维护

介绍PLC运行的环境、日常维护工作和对PLC系统各部件进行安全检测的方法。     

Association between Single Nucleotide Polymorphism rs1044925 and the Risk of Coronary Artery Disease and Ischemic Stroke

The present study was performed to clarify the association between the acyl-CoA:cholesterol acyltransferase-1 (ACAT-1) single nucleotide polymorphism (SNP) rs1044925 and the risk of coronary artery disease (CAD) and ischemic stroke (IS) in the Guangxi Han population. Polymerase chain reaction and restriction fragment length polymorphism was performed to determine the genotypes of the ACAT-1 SNP rs1044925 in 1730 unrelated subjects (CAD, 587; IS, 555; and healthy controls; 588). The genotypic and allelic frequencies of rs1044925 were significantly different between the CAD patients and controls (p = 0.015) and borderline different between the IS patients and controls (p = 0.05). The AC/CC genotypes and C allele were associated with a decreased risk of CAD and IS (CAD: p = 0.014 for AC/CC vs. AA, p = 0.022 for C vs. A; IS: p = 0.014 for AC/CC vs. AA; p = 0.017 for C vs. A). The AC/CC genotypes in the healthy controls, but not in CAD or IS patients, were associated with an increased serum high-density lipoprotein cholesterol (HDL-C) concentration. The present study shows that the C allele carriers of ACAT-1 rs1044925 were associated with an increased serum HDL-C level in the healthy controls and decreased risk in CAD and IS patients.     

Necessary and sufficient convergence conditions of the instrumental variable method for identification

The consistency of the instrumental variable method is considered for a system driven by independent identically distributed input signals with zero mean. Necessary and sufficient conditions for convergence are developed with respect to the given model structure. The sufficient and necessary conditions are established for cases with and without data prefiltering. In this paper we extend on existing results such that the requirements on the degree of the instrumental variables can be relaxed. Numerical examples supporting the theoretical results are provided.     

西气东输江都-如东线网络架构的优化

针对西气东输江都-如东线频繁出现网络故障的情况,基于江都-如东线现有网络架构,分析了网络系统存在的不足,对网络架构进行了优化,重新划分了局域网,处理了网络环网,禁用了路由器的多余广播,进行了风暴控制,优化了ESD系统参数,提出了在江都-如东线进行网络架构优化的方案。通过对思科交换机、思科路由器、SIXNET交换机和ESD系统的配置进行修改,以及为期1年的试运行,实现了网络故障零发生的目标,提高了网络通讯的质量,保证了系统的可靠性和安全性。     

4G环境下集中化业务支撑系统建设探讨

自4G商用以来,各运营商的4G发展遇到诸多挑战。在业务运营支撑方面,电信运营商原有的两级架构属于分散管理,全网业务难以快速推向市场,集中化运营缺乏有效支撑。本文从4G集中化建设目标出发,结合现有两级支撑架构,分析比对了四种集中化业务支撑思路并给出选择建议,对今后电信运营商集中化支撑系统建设具有一定的启发作用。     

Typoselectivity of Crude Geobacillus sp. T1 Lipase Fused with a Cellulose-Binding Domain and Its Use in the Synthesis of Structured Lipids

Typoselectivity of crude CBD-T1 lipase (Geobacillus sp. T1 lipase fused with a cellulose binding domain) was investigated. Multi-competitive reaction mixtures including a set of n-chain fatty acids (C8:0, C10:0, C12:0, C14:0, C18:1 n-9, C18:2 n-6 and C18:3 n-3) and tripalmitin-enriched triacylglycerols were studied in hexane. The crude CBD-T1 lipase discriminated strongly against C18:1 n-9 [competitive factor (α) = 0.23] and showed the highest preference for C8:0 (α = 1). Utilizing the catalytic properties of crude CBD-T1 lipase, acidolysis of soybean oil with C8:0 was selected as a model reaction to investigate the ability of the lipase to produce MLM-type (medium-long-medium) structured lipids. Several reaction parameters (added water amount, reaction temperature, substrate molar ratio and reaction time) examined for incorporating C8:0 into soybean oil, the optimum conditions were: 1:3 (soybean oil/C8:0) of molar ratio, 3 mL of hexane, 50 °C of temperature, 48 h of reaction time, 20 % of crude CBD-T1 lipase (w/w total substrates), and 7.5 % of water (w/w enzyme). Under these conditions, the incorporation of C8:0 was 29.6 mol%. The results suggest that crude CBD-T1 lipase, which showed different fatty acid specificity profiles, is a potential biocatalyst for the modification of fats and oils.